Antimicrobial Testing >> Antibacterial Finishes

AATCC 100 Antibacterial Finishes

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Test Method(s):
    · AATCC 100

AATCC 100, Assessment of Antibacterial Finishes on Textile Materials


This test method provides a quantitative procedure for the evaluation of the degree of antibacterial activity.  Assessment of antibacterial finishes on textile materials is determined by the degree of antibacterial activity intended in the use of such materials.

If only bacteriostatic (inhibition of multiplication) is intended, a qualitative procedure which clearly demonstrates antibacterial activity as contrasted with lack of such activity by an untreated specimen may be acceptable.

If bactericidal activity is intended or implied, quantitative evaluation is necessary.  Quantitative evaluation also provides a clearer picture for possible uses of such treated textile materials.

Test and control swatches are inoculated with the test organisms.  After incubation, the bacteria are eluted from the swatches by shaking in known amount of neutralizing solution. The number of bacteria present in this liquid is determined, and the percentage reduction by the treated specimen is calculated.

Test Organisms:

  • Staphylococcus aureus ATCC 6538
  • Klebsiella pneumoniae ATCC 4352
  • Other suitable speciies can also be used

The number of swatches to be used is dependent on the fiber type and fabric construction. Use that amount of fabric which will absorb 1ml of inoculum, and leave no free liquid in the jar.

In the absence of an untreated negative control, quantitative method AATCC 100 can be run by comparing start and end concentration of sample.