ASTM E2149 Antimicrobial Activity

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ASTM E2149 Antimicrobial Activity

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Test Method(s):
    · ASTM E 2149

ASTM E 2149, Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions


This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions.   This dynamic shake flash test was developed for routine quality control and screening test.

  • Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.
  • The presence of a leaching antimicrobial is determined post-test.


Summary of Test Method:

The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.  The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.  The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls.

Test Organisms: Escherichia coli ATCC 25922

Results can be presented in either percent reduction when measuring CFU/mL or as Log bacterial reduction when calculating mean log density of bacteria.

Record and report presence of solution activity.  Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.