Antimicrobial Testing >> Antimicrobial Activity
ASTM E2149 Antimicrobial Activity
· ASTM E 2149
ASTM E 2149, Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions
This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions. This dynamic shake flash test was developed for routine quality control and screening test.
- Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.
- The presence of a leaching antimicrobial is determined post-test.
Summary of Test Method:
The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls.
Test Organisms: Escherichia coli ATCC 25922
Results can be presented in either percent reduction when measuring CFU/mL or as Log bacterial reduction when calculating mean log density of bacteria.
Record and report presence of solution activity. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.