Antimicrobial Testing >> Parallel Streak Method

Parallel Streak Method AATCC 147

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Test Method(s):
    · AATCC 147

AATCC 147, Antibacterial Activity Assessment of Textile Materials

Scope: The objective is to detect bacteriostatic activity on textile materials. The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting increasing degrees of sensitivity.

  • Specimens of the test materials are placed in intimate contact with the agar surface which has been previously streaked with an inoculum of a test bacterium
  • After incubation, a clear area of interrupted growth underneath and along the side of the test material indicates antibacterial activity of the specimen

Test Organisms: Staphylococcus aureus, ATCC 6538; Klebsiella pneumoniae, ATCC 4352; other suitable species can also be used depending on the intended end-use of the test sample.

Parallel Streak works very well for thin woven fabrics. For materials that roll and/or are bulky in construction, the results of these tests are not always conclusive, since the intimate contact with the agar is not possible. In such cases, a quantitative test will give more accurate results.